How to build a Custom Reference Hashtable for BSSH DRAGEN Single Cell RNA.

DRAGEN uses hashtable references to map sequence reads to a reference genome. FASTA files are used as input for DRAGEN to generate hashtables, which are a custom, binary format of the reference genome that DRAGEN requires. The hashtable used must match the version expected by the DRAGEN Single Cell RNA Analysis pipeline to be run.

Please refer to the table below for compatibility:

	Analysis Setup	Hashtable Version	Hash Table Application
DRAGEN Single Cell 4.4.2	Run Planning	10	Reference Builder (Instruments) v3.0.0
DRAGEN Single Cell 4.4.2	Manual App Launch	10	DRAGEN Reference Builder 4.3.6
DRAGEN Single Cell 4.4.4	Manual App Launch	11	DRAGEN Reference Builder 4.4.4001

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