# Instrument, flow cell, and reagent requirement FAQ for Illumina DNA PCR Free libraries

**Can Illumina DNA PCR-Free Tagmentation libraries be sequenced on all Illumina platforms?**\
This library type is not supported on HiSeq X, but can be sequenced on all other platforms. See the [Custom Primer Requirements for the Illumina DNA PCR-Free Prep, Tagmentation Kit article](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-reference_material-list/000003343) and app note [Optimal loading concentration for Illumina DNA PCR-Free libraries](https://www.illumina.com/content/dam/illumina/gcs/assembled-assets/marketing-literature/illumina-dna-pcr-free-loading-concentration-tech-note-770-2020-007/illumina-dna-pcr-free-loading-concentration-tech-note-770-2020-007.pdf) for more details.

**Which NovaSeq 6000 flow cells can be used with these libraries?**\
All NovaSeq flow cells can be used: SP, S1, S2 and S4.

**If Illumina DNA PCR-Free libraries are sequenced without adding any custom primers, can the flow cell be recovered?**

No.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #3443), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000003443%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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