Instrument, flow cell, and reagent requirement FAQ for Illumina DNA PCR Free libraries
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Can Illumina DNA PCR-Free Tagmentation libraries be sequenced on all Illumina platforms? This library type is not supported on HiSeq X, but can be sequenced on all other platforms. See the Custom Primer Requirements for the Illumina DNA PCR-Free Prep, Tagmentation Kit article and app note Optimal loading concentration for Illumina DNA PCR-Free libraries for more details.
Which NovaSeq 6000 flow cells can be used with these libraries? All NovaSeq flow cells can be used: SP, S1, S2 and S4.
If Illumina DNA PCR-Free libraries are sequenced without adding any custom primers, can the flow cell be recovered?
No.
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