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Chemistry and Imaging on the NovaSeq X/X Plus Instruments

The NovaSeq X/X Plus is powered by XLEAP-SBS chemistry, a faster, higher quality, and more robust update to proven Illumina sequencing by synthesis (SBS) chemistry.
Chemistry
The NovaSeq X/X Plus uses two-color chemistry, which allows for very fast imaging of the flow cell using blue and green channels at every sequencing cycle.
  • Clusters with intensity in green only: T
  • Clusters with intensity in blue only: A
  • Clusters with intensity in both channels: C
  • Clusters with no intensity/dark: G
Please note the following differences between the NovaSeq X series and other two-channel Illumina platforms:
  • The NovaSeq 6000, NextSeq 500/550, and MiniSeq: A channel uses combined Red and Green, and the C channel uses Red only.
  • The NextSeq 1000/2000: A channel uses combined Blue and Green, and the C channel uses Blue only
10B Flow Cell Layout There are2 swaths per each surface (2 top and 2 bottom), 2 surfaces per lane, and 98 tiles per swath for a total of 3136 tiles imaged.
25B Flow Cell Layout
There are 4 swaths per each surface (4 top and 4 bottom), 2 surfaces per lane, and 98 tiles per swath for a total of 6272 tiles imaged.
For any feedback or questions regarding this article (Illumina Knowledge Article #7970), contact Illumina Technical Support [email protected].
Last modified 25d ago
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