How to Configure Network Settings for the MiSeq i100 after First Time Setup

Background

The MiSeq i100 Series can be configured to connect to a local network to enable the instrument to output data to local network attached storage, as well as upload data to BaseSpace and Proactive if it has an active internet connection.

The First Time Setup workflow walks users through the process step-by-step, though this process is optional and can be skipped during First Time Setup. The following workflow assists users in using the Settings menu to connect their instrument to their network outside of the First Time Setup workflow.

Connecting to the Network

1. Connect the supplied ethernet cable to the LAN1 ethernet port on the back of the instrument.

2. Login to the MiSeq i100 Control Software as an Administrator user.

3. From the Home screen, select the dropdown menu in the top-left corner and select Settings, then select Network Settings.

4. Select the Edit button next to LAN1 to configure the primary network connection.

5. In the Network Settings: LAN1 user interface (UI), locate sections for configuring the IP address on the left and DNS Server on the right.

6. For configuring the IP Address settings on the left:

   1. The default selection is Automatically assign IP address (DHCP). If the network is configured to allow DHCP connections, the instrument automatically populates the IP Address, Netmask, and Gateway fields after a couple minutes.

   2. If the network uses Static IP assignments, select the radio button for 'Manually enter IP address' and then input the IP Address, Netmask, and Gateway information.

      1. These details must be provided by the site's IT team and cannot be provided by Illumina Support.

7. For configuring DNS Servers on the right:

   1. The default selection is Automatically assign DNS server IP address. If the network is configured to automatically detect DNS IP addresses, the instrument automatically populates the DNS server(s) IP address field after a couple minutes.

   2. If the network is not configured to provide DNS server IP addresses automatically, select the radio button for Manually enter DNS server IP address and then input the IP Address for each DNS server separated by a comma.

   3. If the network uses a local network domain name (ie company.com), input this domain in the Search domain box.

   4. These details must be provided by the site's IT team and cannot be provided by Illumina Support.

8. Select Save to save the LAN1 configuration settings.

This configures the primary network settings and allows the MiSeq i100 Series instrument to connect to the local network. If the network is configured to allow internet access, the instrument can now activate Proactive and BaseSpace connections through the Cloud Settings menu.

See MiSeq i100 Series Product Documentation - Cloud Settings for additional details on configuring Cloud Settings.

See the sections below for instructions to configure the instrument to connect through a Proxy computer or enable the inbound Firewall ports for Illumina Run Manager if needed.

Configure Proxy Settings

This section describes how to configure the instrument to connect to a proxy computer on the local network. This is only required if a proxy computer is used on the local network, consult local IT team for guidance.

1. Select the menu icon in the upper-left corner.

2. Select Settings, and then select Proxy Settings.

3. Select the checkbox next to Enable proxy.

   1. Enter the Server address.

   2. [Optional] Enter the Port.

4. [Optional] If needed, select Requires user name and password.

   1. Enter the User name used to authenticate with the Proxy server.

   2. Enter Password for the User name.

5. Select Save to finish configuring the Proxy Settings.

Configuring the Inbound Firewall Ports

The Illumina Run Manager interface allows users to use a web browser to setup sequencing runs, requeue analysis, and other tasks on the instrument from a separate computer on the same network. To allow these connections, the MiSeq i100 Control Software must be configured to allow inbound connections on ports 80 and 443.

See the instructions below to perform this configuration.

1. Select the menu icon in the upper-left corner.

2. Select Settings, and then select Firewall.

3. Select the checkbox to enable ports 80 and 443 for remote access.

4. Select Save to finish configuring the Firewall settings.

For any feedback or questions regarding this article (Illumina Knowledge Article #9308), contact Illumina Technical Support [e-mail].