# How to Configure Network Settings for the MiSeq i100 after First Time Setup **Background** The MiSeq i100 Series can be configured to connect to a local network to enable the instrument to output data to local network attached storage, as well as upload data to BaseSpace and Proactive if it has an active internet connection. The First Time Setup workflow walks users through the process step-by-step, though this process is optional and can be skipped during First Time Setup. The following workflow assists users in using the Settings menu to connect their instrument to their network outside of the First Time Setup workflow. *Connecting to the Network* 1. Connect the supplied ethernet cable to the **LAN1** ethernet port on the back of the instrument. 2. Login to the MiSeq i100 Control Software as an **Administrator** user. 3. From the Home screen, select the dropdown menu in the top-left corner and select **Settings**, then select **Network Settings**. 4. Select the **Edit** button next to **LAN1** to configure the primary network connection. 5. In the **Network Settings: LAN1** user interface (UI), locate sections for configuring the **IP address** on the left and **DNS Server** on the right. 6. For configuring the **IP Address** settings on the left: 1. The default selection is **Automatically assign IP address (DHCP)**. If the network is configured to allow DHCP connections, the instrument automatically populates the IP Address, Netmask, and Gateway fields after a couple minutes. 2. If the network uses **Static IP** assignments, select the radio button for 'Manually enter IP address' and then input the **IP Address**, **Netmask**, and **Gateway** information. 1. These details must be provided by the site's IT team and cannot be provided by Illumina Support. 7. For configuring **DNS Servers** on the right: 1. The default selection is **Automatically assign DNS server IP address**. If the network is configured to automatically detect DNS IP addresses, the instrument automatically populates the DNS server(s) IP address field after a couple minutes. 2. If the network is not configured to provide DNS server IP addresses automatically, select the radio button for **Manually enter DNS server IP address** and then input the **IP Address** for each DNS server separated by a comma. 3. If the network uses a local network domain name (ie company.com), input this domain in the **Search domain** box. 4. These details must be provided by the site's IT team and cannot be provided by Illumina Support. 8. Select **Save** to save the LAN1 configuration settings. This configures the primary network settings and allows the MiSeq i100 Series instrument to connect to the local network. If the network is configured to allow internet access, the instrument can now activate Proactive and BaseSpace connections through the Cloud Settings menu. See [MiSeq i100 Series Product Documentation - Cloud Settings](https://support-docs.illumina.com/IN/MiSeqi100Series/Content/IN/MiSeqi100/SysBSSH.htm) for additional details on configuring Cloud Settings. See the sections below for instructions to configure the instrument to connect through a **Proxy** computer or enable the inbound **Firewall** ports for Illumina Run Manager if needed. *Configure Proxy Settings* This section describes how to configure the instrument to connect to a proxy computer on the local network. This is only required if a proxy computer is used on the local network, consult local IT team for guidance. 1. Select the menu icon in the upper-left corner. 2. Select **Settings**, and then select **Proxy Settings**. 3. Select the checkbox next to **Enable proxy**. 1. Enter the **Server address**. 2. \[Optional] Enter the **Port**. 4. \[Optional] If needed, select **Requires user name and password**. 1. Enter the **User name** used to authenticate with the Proxy server. 2. Enter **Password** for the User name. 5. Select **Save** to finish configuring the **Proxy Settings**. *Configuring the Inbound Firewall Ports* The Illumina Run Manager interface allows users to use a web browser to setup sequencing runs, requeue analysis, and other tasks on the instrument from a separate computer on the same network. To allow these connections, the MiSeq i100 Control Software must be configured to allow inbound connections on ports 80 and 443. See the instructions below to perform this configuration. 1. Select the menu icon in the upper-left corner. 2. Select **Settings**, and then select **Firewall**. 3. Select the checkbox to **enable ports 80 and 443 for remote access**. 4. Select **Save** to finish configuring the **Firewall** settings. ## *Set Default Output Folder* To use an external storage option as the default output folder, select the external storage output folder as follows. 1. Select the menu icon in the upper-left corner. 2. Select **Settings**, and then select **External storage**. 3. If an output folder has already been added, select **Edit folders**, and then select **Add folder**. 4. If an output folder has not been added, select **Add folder**.\ The folder name cannot contain a space. 5. Select a server location from the drop-down list, and then select one of the available volumes. 6. Select the desired default output folder from Available folders. 7. \[Optional] Enter a folder nickname. 8. Select **Save**. 9. To remove output folders, select **Remove** on the Edit folders screen. \ \ \
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