TruSeq Stranded RNA Kit FAQs: AMPure clean up and library size

What do I do if my AMPure beads are not dry after the 15 minutes incubation at room temp? Longer drying times may be required depending on environmental variables and the amount of ethanol remaining in the well. However, take care not to over-dry beads as this can impact sample recovery. Fewer than 15 minutes may be sufficient for drying.

What is the size of adapter dimers with the dual indexed adapters? The adapter dimers will be approximately 136 bp, may run closer to 150 bp on a trace.

Can I generate larger RNA fragment sizes? It is possible to use larger fragment sizes, however, we have found that shorter fragment sizes generate the best coverage. Please refer to the Appendix section in the Reference Guide for more information.

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