Flow cell is not detected error on the NextSeq 1000/2000

Background: When starting a run, the NextSeq 1000/2000 can present an error that the "flow cell is not detected" despite a properly loaded flow cell.

To troubleshoot:

1. Investigate the flow cell seating and cleanliness.

• Retry by reseating the flow cell.

2. Power cycle\* the system.

• Confirm failure to recognize the flow cell persists through the power cycle.

• \*For further information, see the Knowledge Base articles Power Cycling on the NextSeq 1000/2000 andPower Cycling on the NextSeq 1000/2000 Video.

3. If issue persists, prepare the requested information below and contact Illumina Technical Support for further guidance.

When reaching out to Illumina Technical Support, provide the following:

• Control Software Logs: /usr /local/Illumina/Logs/nextseq1k2k-control-software

• Service Software Logs: /usr /local/Illumina/Logs/nextseq1k2k-service-software

For any feedback or questions regarding this article (Illumina Knowledge Article #6639), contact Illumina Technical Support [e-mail].