> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000003448.md).

# Consumables used in Illumina DNA PCR-Free workflow

**Is there a document describing everything included in the kit?**\
Refer to the Product Contents section of the [Reference Guide](https://support.illumina.com/downloads/illumina-dna-pcr-free-reference-guide.html).

**Which user**-**supplied consumables**, **plastics**, **and benchtop accessories are needed?**\
Refer to the [Reference Guide's](https://support.illumina.com/downloads/illumina-dna-pcr-free-reference-guide.html) User-Supplied Consumables & Equipment section.

**Which pipette tips are recommended?**\
Filter DNase/RNase free low-binding tips.

**Can strip tubes be used instead of PCR plates?**\
Strip tubes will work for reactions provided that the thermal cycler can fit them adequately and that the correct magnet is utilized. An example of a magnet that works with strip tubes is the DynaMag-96 Side Magnet (Cat. # 12331D) from Thermo Fisher Scientific.

**What are the consequences of using a different type of plate for reactions in thermal cycler and incubator protocols?**

* **The thermal cycler protocol uses PCR plates**: PCR plates different than recommended will need to be evaluated for several factors, such as volume held in wells and type of magnet needed. The protocol is effective when using 96-well hard-shell plates from BioRad (HSP-9601) and DynaMag-96 Side Skirted Magnet (Cat. # 12027). Different magnet/plate combinations may necessitate more careful supernatant removal if magnet concentrates beads at the bottom of the well.
* **The incubator protocol uses MIDI plates**: MIDI plates other than recommended must be evaluated for several factors, such as volume held in wells and type of magnet needed. The protocol is effective when Amgen MIDI plates (Cat. # AB-0859) and Magnetic Stand-96 (Cat. # AM10027) are used. Due to MIDI plate design, pipetting steps are substituted with shaking steps, and incubation steps duration and temperatures are adjusted to take into account heat distribution, due to material differences (thickness of the plastic and mass) compared to PCR plates.

**Can other kinds of plate seals be used instead of Microseal B adhesive seals?**

Use Microseal 'B' adhesive seals for shaking, centrifuging, and long-term storage. The seals are effective at -40°C to 110°C and suitable for skirted or semiskirted PCR plates.

Microseal 'F' adhesive foils are effective at temperatures as low as -70°C and suitable for longterm storage of the 96-well plate containing final libraries.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #3448), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000003448%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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