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General information on Ribo Zero Plus Microbiome
What is Ribo-Zero Plus Microbiome?* This product contains additional rRNA depletion probes, designed to target species found in the microbiome of human adult and infant stool samples.
- Ribo-Zero Plus Microbiome can either be purchased as a standalone product, or bundled with library prep reagents in the Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus Microbiome kit, listed on the product page here.
Which other sample types are compatible with Ribo-Zero Plus Microbiome?* Illumina has reports of effective depletion from skin, vaginal, and oral samples in addition to stool samples, though results may vary.
What probes are included in Ribo-Zero Plus Microbiome?* The Microbiome probe set includes a total of 1092 probes, including ATCC probes (380), and the existing E. coli and B. subtilis probes (57).
How much input is needed? What quality does it need to be? * The Ribo-Zero Plus Microbiome protocol is optimized for 25-500 ng of total RNA input.
- RNA is recommended to be at least RIN â‰¥ 5.
How to use the Ribo-Zero Plus Microbiome reagents?* The full protocol is in the updated Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus, Ribo-Zero Plus Microbiome Reference Guide (1000000124514 v03 or later).
- Briefly, DPM (Microbiome Depletion Probe Pool) is added to DP1 (Ribo-Zero Plus Depletion Probe Pool) to deplete all rRNA species in a single step.
What are the sequencing recommendations for Ribo-Zero Plus Microbiome?* Illumina recommends a 2 x 150 bp read length, rather than 2 x 75 bp, for better analysis results.
How to analyze Ribo-Zero Plus Microbiome sequencing data?* One option it to use the Microbiome Metatranscriptomics App In BaseSpace Sequence Hub.
- This app is optimized for data prepared using the Illumina Stranded Total RNA with Ligation, Ribo-Zero Plus Microbiome.