# Representative library QC traces for Illumina Single Cell 3’ RNA Prep
**The final Illumina Single Cell 3' RNA Prep library distribution and average fragment size may vary based on sample type and experimental condition(s)**.
The following TapeStation-generated traces serve as representative examples of satisfactory library QC profiles (from satisfactory [cDNA QC](/library-preparation/illumina-single-cell-rna-prep/library-preparation-illumina-single-cell-rna-prep-reference_material-list/000009524.md)) generated during the "Check Library" step of the Illumina Single Cell 3' RNA Prep workflow. For more information, see the appropriate reference guide.
**Human peripheral blood mononuclear cells (PBMCs)**
**Human HEK 293T & mouse NIH 3T3 cell line mixture**
**Mouse frozen tissue**: **unfixed brain nuclei**
**Mouse frozen tissue**: **DSP-methanol fixed brain nuclei**
**Mouse frozen tissue: unfixed lung nuclei**
Refer to [Assessing library QC in Illumina Single Cell 3’ RNA Prep](https://github.com/illumina-swi/illumina-knowledge/blob/master/articles/Knowledge/Assessing-library-QC-in-Illumina-Single-Cell-3-RNA-Prep/README.md) and the [Illumina Single Cell 3' RNA Prep Training Packet](https://support.illumina.com/sequencing/sequencing_kits/illumina-single-cell-prep.html) for further guidance and [library QC best practices.](/library-preparation/illumina-single-cell-rna-prep/library-preparation-illumina-single-cell-rna-prep-reference_material-list/000009526.md)
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| *For any feedback or questions regarding this article (Illumina Knowledge Article #9528), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000009526%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |
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