> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000005467.md).

# Is the Nextera TDE1 enzyme pre loaded with oligos?

The Illumina transposase enzymes function by inserting a piece of DNA at the site that is cleaved/fragmented by the enzyme in a process called tagmentation.

The transposase enzyme in complex with the piece of DNA that will be inserted is referred to as a 'transposome'.

**All Illumina tagmentation-based library prep kits** (including Illumina Tagment DNA TDE1 Enzyme and Buffer Kits) **come with transposase that is pre-loaded with partial adapter sequences.**

The sequences of the pre-loaded adapters are provided in the [Illumina Adapter Sequences Document](https://support.illumina.com/downloads/illumina-adapter-sequences-document-1000000002694.html) in the 'Nextera Transposase Adapters' section:

![](/files/g12WyFqRRaUgOOmHimhq)

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #5467), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000005467%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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