Is the Nextera TDE1 enzyme pre loaded with oligos?
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The Illumina transposase enzymes function by inserting a piece of DNA at the site that is cleaved/fragmented by the enzyme in a process called tagmentation.
The transposase enzyme in complex with the piece of DNA that will be inserted is referred to as a 'transposome'.
All Illumina tagmentation-based library prep kits (including Illumina Tagment DNA TDE1 Enzyme and Buffer Kits) come with transposase that is pre-loaded with partial adapter sequences.
The sequences of the pre-loaded adapters are provided in the Illumina Adapter Sequences Document in the 'Nextera Transposase Adapters' section:
For any feedback or questions regarding this article (Illumina Knowledge Article #5467), contact Illumina Technical Support .
