# Troubleshooting "Would you like to include the first time setup results…?" on NextSeq 500/550 The Windows 10 upgrade for the NextSeq 500/550 adds a feature that allows the 'instrument setup .csv' file to be added to the run folder.\* The 'instrumentsetup.csv' file contains information from the initial instrument set up. * This message and file have no impact on runs regardless of the choice that is chosen. Upon run completion, NextSeq Control Software shows the message: * "Your input is needed. Would you like to include the first-time setup results with this run?" * The options given are: 'Yes', 'No', and 'Don't ask again.' This message is expected and does not affect sequencing. * If the user selects 'Yes', the 'instrumentsetup.csv' file will be added to the run folder; the instrument will proceed to the post-run wash, and the message will show up again on the next run. * If the user selects 'No' the 'instrumentsetup.csv' file will not be added to the run folder; the instrument will proceed to the post-run wash, and the message will show up again on the next run. * If the user selects 'Don't ask again' the prompt will not show up again and the instrument will proceed to the post-run wash. \ \ \
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