How to power cycle the NovaSeq X Series Instruments

Background

Restarting or power cycling the NovaSeq X or X Plus can resolve many common communication or instrumentation issues. However, these actions should only be performed when recommended by Illumina Tech Support or Field Service.

If the NovaSeq X Series Control Software is not accessible or not responding, do not shutdown through the Linux Operating System (OS) and contact Illumina Tech Support for further guidance.

Restarting the Single Board Computer (SBC):

1. From the NovaSeq X Control Software Home screen, select the instrument icon to open the global navigation menu, and then select Settings.

2. From the Settings menu, select Exit Software.

3. Within the Linux OS, select Activities in the top left corner, then select the drop down menu in the top-right corner.

4. Select the Power icon, then select Restart.

   1. Do not select Power Off as this can prevent the SBC from restarting as expected. If Power Off is selected, contact Illumina Tech Support for further guidance.

5. Once the SBC has restarted, login to the Linux Operating System (OS) and then restart the NovaSeq X Series Control Software.

Power Cycling the NovaSeq X Series Instruments:

For Control Software v1.2.2 and above

For instruments running Control Software v1.2.2 and above, perform the steps listed below every 14 days with the regularly scheduled Maintenance Wash.

1. From the NovaSeq X Control Software Home screen, select the instrument icon to open the global navigation menu, and then select Settings.

2. From the Settings menu, select Shutdown.

3. DO NOT toggle the power switch on the back of the instrument, the instrument will fully power down on it's own.

4. Wait until the power button on the right side of the instrument begins to pulse, then press the power button.

   1. This step can take up to 25 minutes.

5. Allow the instrument to boot, then login to the Linux OS.

6. Once logged into the Linux OS, select Activities in the top-left corner, then select the NovaSeq X Series Control Software icon to launch the Control Software.

   1. Instrument boot and Initialization can take 20-25 minutes in total.

For Control Software v1.2 and below:

1. From the NovaSeq X Control Software Home screen, select the instrument icon to open the global navigation menu, and then select Settings.

2. From the Settings menu, select Shutdown.

3. Wait at least 10 minutes for the instrument to completely shutdown. The monitor should be dark, with no text or cursors flashing in the top left corner.

4. When the instrument computer has powered down and the monitor is dark, toggle the power switch on the back right side of the instrument to the OFF position.

5. Allow the instrument to remain powered down for 30 seconds.

6. Toggle the power switch on the back of the instrument to the ON position.

7. Wait until the power button on the right side of the instrument begins to pulse, then press the power button.

8. Allow the instrument to boot, then login to the Linux Operating System (OS).

9. Allow 5 minutes for the instrument Compute Engine (CE) and Kubernetes microservices to initialize before attempting to start the Control Software.

10. Within the Linux OS, select Activities in the top left corner, then select the NovaSeq X Series Control Software icon and allow control software to initialize.

• The Windows key on the keyboard can be used to open the Activities menu and select the NovaSeq X Series Control Software icon.

If the NovaSeq X Series Control Software does not initialize as expected after restarting the SBC or power cycling the instrument, contact Illumina Tech Support for further guidance.

For any feedback or questions regarding this article (Illumina Knowledge Article #7478), contact Illumina Technical Support [e-mail].