# Troubleshooting RFID errors on NextSeq 500/550

These types of errors can occur during run setup when consumables are loaded into the instrument. These can be due to a transient communication issue with the Radio-frequency identification (RFID) sensor, a broken sensor, or a fault in the consumable RFID.

Unlike the MiSeq, there is no bypass code that can be used as an option to bypass the error on the NextSeq 500/550.

\*\*Error message:\*\*RFID tag appears to be blank: signed data layout version = 0\
![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-286d64cb097ec32015dbe8d1837179ed6f2386d7%2Fimage1.png?alt=media\&token=159a2534-7135-48f6-801e-a7e0cd23c647)

The error may appear as: "***Cannot read information off inserted buffer bottle***"

![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-e7bd7023201bd310caa4cc18215a7aae2a3b4b82%2Fimage2.jpg?alt=media\&token=c5529c0c-7d94-44e6-9e2c-cd7c7b15bca9)

This second error may occur with the buffer cartridge, as sometimes during loading, the NextSeq 500/550 may read the wash buffer cartridge present in the instrument after loading the flow cell.

**Troubleshooting steps:**

1. Re-seat/reinsert consumable.
2. Power cycle\* the instrument.
3. If issue persists, try a new consumable.

* If resolved, it is most likely an RFID issue with the consumable. Contact [Illumina Technical Support](https://www.illumina.com/company/contact-us.html#/united-states/technical-support) with the serial/lot number of the reagent to request a replacement.

4. If steps 1-3 do not resolve the issue, this indicates an issue with the RFID sensor on the instrument. Contact [Illumina Technical Support](https://www.illumina.com/company/contact-us.html#/united-states/technical-support) for further assistance.

\*For further information, search for Knowledge Base article **How to perform a NextSeq 550 Power Cycle.**

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #1914), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000001914%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |