Troubleshooting RFID errors on NextSeq 500/550
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These types of errors can occur during run setup when consumables are loaded into the instrument. These can be due to a transient communication issue with the Radio-frequency identification (RFID) sensor, a broken sensor, or a fault in the consumable RFID.
Unlike the MiSeq, there is no bypass code that can be used as an option to bypass the error on the NextSeq 500/550.
**Error message:**RFID tag appears to be blank: signed data layout version = 0
The error may appear as: "Cannot read information off inserted buffer bottle"
This second error may occur with the buffer cartridge, as sometimes during loading, the NextSeq 500/550 may read the wash buffer cartridge present in the instrument after loading the flow cell.
Troubleshooting steps:
Re-seat/reinsert consumable.
Power cycle* the instrument.
If issue persists, try a new consumable.
If resolved, it is most likely an RFID issue with the consumable. Contact Illumina Technical Support with the serial/lot number of the reagent to request a replacement.
If steps 1-3 do not resolve the issue, this indicates an issue with the RFID sensor on the instrument. Contact Illumina Technical Support for further assistance.
*For further information, search for Knowledge Base article How to perform a NextSeq 550 Power Cycle.
For any feedback or questions regarding this article (Illumina Knowledge Article #1914), contact Illumina Technical Support techsupport@illumina.com.
