Troubleshooting RFID errors on NextSeq 500/550

These types of errors can occur during run setup when consumables are loaded into the instrument. These can be due to a transient communication issue with the Radio-frequency identification (RFID) sensor, a broken sensor, or a fault in the consumable RFID.

Unlike the MiSeq, there is no bypass code that can be used as an option to bypass the error on the NextSeq 500/550.

**Error message:**RFID tag appears to be blank: signed data layout version = 0

The error may appear as: "Cannot read information off inserted buffer bottle"

This second error may occur with the buffer cartridge, as sometimes during loading, the NextSeq 500/550 may read the wash buffer cartridge present in the instrument after loading the flow cell.

Troubleshooting steps:

1. Re-seat/reinsert consumable.

2. Power cycle\* the instrument.

3. If issue persists, try a new consumable.

• If resolved, it is most likely an RFID issue with the consumable. Contact Illumina Technical Support with the serial/lot number of the reagent to request a replacement.

4. If steps 1-3 do not resolve the issue, this indicates an issue with the RFID sensor on the instrument. Contact Illumina Technical Support for further assistance.

\*For further information, search for Knowledge Base article How to perform a NextSeq 550 Power Cycle.

For any feedback or questions regarding this article (Illumina Knowledge Article #1914), contact Illumina Technical Support [e-mail].