Troubleshooting Fluidics Pre Run Check Errors at 88% on the MiSeq i100 Series

Background

During the Pre-Run Checks, the instrument measures the average Illumination intensity after flowing buffer through the flow cell to ensure that the Optics and Fluidics systems are performing as expected.

If the average intensity values are outside of specification, the Pre-Run Checks stop during the Fluidics checks approximately 88% of the way through the check completion. If this Issue occurs, please see the following Troubleshooting Actions

Troubleshooting Actions

1. Cancel the Pre-Run Checks and exit the Run Setup.

2. Power cycle the instrument.

   1. See Knowledge Article How to Power Cycle the MiSeq i100 Series for additional details if needed.

3. Perform the instrument System Health Checks.

   1. See Knowledge Article How to Setup the System Health Checks on the MiSeq i100 Series Instruments for additional details if needed.

4. Collect the Small Export Logs bundle.

   1. See Knowledge Article How to Export Logs on the MiSeq i100 Series for additional details if needed.

5. Contact Illumina Technical Support to review the Pre-Run Check results and System Health Check report before proceeding with follow up sequencing run.

For any feedback or questions regarding this article (Illumina Knowledge Article #9693), contact Illumina Technical Support [email protected].