# Indexed Sequencing workflows with 5' to 3' directionality
**Single-Indexed Sequencing**The single-indexed sequencing workflow applies to all Illumina sequencing platforms, where an Index Read follows Read 1.
**Dual-Indexed Workflow on a Paired-End Flow Cell**
**Forward Strand Workflow:** MiniSeq with Rapid Reagent kits and the MiSeq.
**Note**: While MiniSeq Rapid reagents allow for dual indexing, Read 2 cannot be performed with the MiniSeq Rapid reagent kit.
**Reverse Complement Workflow:** iSeq 100, MiSeq i100 (read-first sequencing), MiniSeq with Standard reagent kits, NextSeq 500/550, NextSeq 1000/2000, NovaSeq 6000 with v1.5 reagent kits, and NovaSeq X Series.
For more information, see the [Indexed Sequencing on Illumina Systems Guide](https://support.illumina.com/downloads/indexed-sequencing-overview-15057455.html?langsel=/us/).
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