Troubleshooting a Failed Camera Board on the NextSeq 500/550

The following error can occur at the very beginning of a sequencing run or while the run is progressing and will cause the run to stop.

The error message states: "All cameras disabled Originally caused by: Failed Camera Board."

Possible causes:

• Transient communication issues.

• A loose connection from the camera board.

• A hardware failure at Main Printed circuit Assembly (MPCA).

Troubleshooting steps:

1. Power cycle the NextSeq 500/550.\*

2. If the system initializes without errors, run the Flow Cell Registration Test.

   1. Navigate to Manage Instrument > System Check, and select the Flow Cell Registration Test.

      1. If the flow cell registration test passes without errors, proceed with setting up the next run and monitor the run performance.

      2. If a similar error appears at any stage, contact Illumina Technical Support.

\*For further information, search for Knowledge Base article How to Perform a NextSeq 500/550 Power Cycle.

For any feedback or questions regarding this article (Illumina Knowledge Article #1683), contact Illumina Technical Support [e-mail].