Bead types in Illumina library preparation kits

Illumina library preparation kits use various bead types for different functions. While these beads often look similar, it is important to use the correct beads when called for because they have different properties and functions. All beads should be:

• Brought to room temperature before use (for approximately 30 minutes)

• Pulse-vortexed for 1 full minute to ensure homogenous resuspension

• Stored at the appropriate temperature when not in use. Storage requirements for each bead type can be found in the library preparation reference guide.

The tables below list bead types by function and provide additional information about each bead type.

DNA Purification

RNA Isolation and Purification

Tagmentation

Enrichment

Normalization

For any feedback or questions regarding this article (Illumina Knowledge Article #3342), contact Illumina Technical Support [e-mail].