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Troubleshooting FPGA RFID ERROR STATUS 2 errors on the MiSeq

The FPGA mediates communication between subsystems of the MiSeq. * If communication is lost with a subsystem, MiSeq Control Software (MCS) will show an error message.
  • For the FPGA RFID ERROR STATUS 2 message, the error may occur if the PR2 bottle is removed from the instrument before "start wash" is selected.
  • This error can indicate communication was lost or an issue with the instrument hardware (radio-frequency identification (RFID) reader).
Error: The FPGA reported an error while executing command "RFID3BLKWR 1 19 80000002"; error message: "RFID ERROR STATUS 2 A00000" (Illumina.Instrument.Bolt.Logic.Mainboard.FPGAErrorResponseException)
To troubleshoot:
  1. 1.
    Verify run completed successfully.
  2. 2.
    Complete a post-run wash if it has not been completed.
  3. 3.
    Power cycle* MiSeq.
  4. 4.
    If the instrument initializes without error, continue using the MiSeq for sequencing.
  5. 5.
    If the error persists, contact Illumina Technical Support for assistance.
*For further information, search for Knowledge Base articles How to Shut down or power cycle the MiSeq and How To Power Cycle the MiSeq Video.
For any feedback or questions regarding this article (Illumina Knowledge Article #1909), contact Illumina Technical Support [email protected].
Last modified 11d ago
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