# Sequencing run setup software considerations FAQ for Illumina DNA PCR Free libraries

What **software is required and available to support sequencing Illumina DNA PCR-Free?**

* Library prep kit definition files for Illumina DNA PCR-free libraries are available for download for use with **Local Run Manager** (LRM) v2. Note that **manual** selection of the custom primers is required in the run setup in the Advanced Options.
* **Illumina Experiment Manager** (IEM) v1.19.1 has been updated to include the Illumina DNA PCR-Free Prep, Tagmentation kit as an available library prep kit.
* See [Custom Primer Requirements for the Illumina DNA PCR-Free Prep, Tagmentation Kit](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-reference_material-list/000003343) for further details on software setup.

**What is the recommended read length?**

* Paired End (Read 1, Index 1, Index 2, Read 2):
  * For MiniSeq and NextSeq 500/550: 149 bp, 10 bp, 10 bp, 149 bp
  * For all other compatible platforms: 151 bp, 10 bp, 10 bp, 151 bp

**What is the process to set up a sample sheet for sequencing Illumina DNA PCR-Free libraries?**

* IEM 1.19 has been updated to support sample sheet creation for Illumina DNA PCR-Free libraries.
* Select the appropriate library preparation and index kits and complete required information.

**What is the process to set up a run in Local Run Manager?**\
See full instructions in the [Custom Primer Requirements for the Illumina DNA PCR-Free Prep, Tagmentation Kit](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-reference_material-list/000003343).

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #3441), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000003441%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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