Viewing NextSeq 1000/2000 runs in Sequencing Analysis Viewer
Last updated
Last updated
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Sequencing Analysis Viewer (SAV) v3.0 has Windows, Mac, and Linux versions; however, it is not compatible with installing on the NextSeq 1000/2000 instrument itself. For more information, see Knowledge article Common Questions Related to Sequencing Analysis Viewer (SAV) software and the NextSeq 1000/2000.
The %Occupied metric has not been optimized for the reduced nanowell pitch width on the NextSeq 1000/2000. %Occupied is not a reliable indicator of flow cell loading on the NextSeq 1000/2000.
%Loading concentration can be used as a gauge for how loaded the flow cell is. For more information on %loading concentration see Knowledge Base Optimizing loading concentration on the NextSeq 1000/2000.
**Schematic of Flow Cells with tiles vs SAV
P1 Flow cell**
P2 Flow cell
P3 Flow cell
Tile Naming Convention:
The tile name is a four-digit number that represents the tile position on the flow cell. For example, tile name 1205 indicates top surface, swath 2, tile 05.
The first digit represents the surface: 1 for top or 2 for bottom.
The second digit represents the swath number: 1, 2, 3, 4, 5, or 6.
The last two digits represent the tile number. For swath numbers 1-4, the numbering starts with 01 at the outlet end of the flow cell through 11 at the inlet end. For swath numbers 5-6, the numbering starts with 01 at the inlet end and 11 at the outlet end.
Version 2.5.12 of Sequencing Analysis Viewer is required for reviewing runs generated on NextSeq 1000/2000 control software version 1.5 or later. The flow cells for the P1 and P2 600 cycle kits have not changed, but the way the RunInfo and ErrorMetrics are loaded in SAV has been updated. SAV v2.5.12 is only validated for use with NextSeq 1000/2000 and is not required for any other platform.
Example of Error displayed when loading run from NextSeq 1000/2000 control software v1.5 into SAV version 2.4.7 or earlier.
For any feedback or questions regarding this article (Illumina Knowledge Article #2501), contact Illumina Technical Support techsupport@illumina.com.
