Troubleshooting Local Run Manager originally caused by an internal server error on NextSeq 500/550

The “Local Run Manager: Originally caused by an internal server error” notification in the NextSeq 500/550 control software can indicate either a transient communication issue or that the PostgreSQL service is not running.

Troubleshooting steps:

1. Power cycle. If control software reinitializes without errors, continue with next sequencing run.

• For additional information, search for knowledge article How to Perform a NextSeq 500/550 Power Cycle.

1. If the notification persists in control software after power cycle, restart the PostgreSQL service with the following steps.

   1. Close the control software.

   2. Search for the Services App in Windows task bar, then right-click and select Run as Administrator.

   3. Search for PostgreSQL and check to see if it's 'Running'. If not, right click and select Start.

   4. Relaunch the control software.

If the issue persists, contact Illumina Technical Support for additional assistance.

For any feedback or questions regarding this article (Illumina Knowledge Article #7806), contact Illumina Technical Support [e-mail].