# NextSeq 500/550

- [FAQ](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list.md)
- [At which cycle of NextSeq 500/550 are run metrics calculated?](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002584.md)
- [Can the NextSeq 500/550 v2.5 flow cell be used with the v2 Reagent cartridge and buffer cartridge?](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002613.md)
- [Can the output folder for a NextSeq 500/550 run be saved to the local computer?](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000005512.md)
- [Common Questions Related to NextSeq 500/550 v2.5 Kit](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002080.md)
- [Common questions regarding custom recipes for the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002618.md)
- [Do NextSeq 500/550 v2.5 runs have different specifications than v2.0 reagent runs?](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002609.md)
- [How long does a NextSeq 500/550 system check take to complete?](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002582.md)
- [How long does it take to purge all reagents in a NextSeq 500/550 run?](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002581.md)
- [How many extra cycles are included in NextSeq 500/550 reagent cartridges?](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002580.md)
- [How to set up a NextSeq 500/550 to upload to a BaseSpace Enterprise account using NCS v.4.0.1](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000005913.md)
- [Run setup modes for the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000005574.md)
- [What do the NextSeq 500/550 status bar colors mean?](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000006206.md)
- [What is the difference between NextSeq PhiX Control Kit and PhiX Control v3?](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002925.md)
- [What is the maximum read length supported in one direction on a NextSeq 500/550 run?](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002588.md)
- [Will the NextSeq 500/550 Control Software prevent run setup with read lengths fewer than 25 cycles?](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002565.md)
- [Reference Material](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list.md)
- [Best Practices for maintaining the computer on a NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000007445.md)
- [Chemistry and imaging on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000002078.md)
- [Consumable part numbers for the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000001975.md)
- [File paths for the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000002072.md)
- [Frequently asked questions about upgrading to NextSeq 500/550 Control Software version 4.0](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000007485.md)
- [Hardware overview for the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000002073.md)
- [How to Denature and Dilute Libraries on the NextSeq 500/550 Video](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000005863.md)
- [How to Find the Control Software Version on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000009328.md)
- [How to Perform a NextSeq 500/550 Power Cycle](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000001972.md)
- [How to Prepare Reagents Pre Run on the NextSeq 500/550 Video](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000007450.md)
- [How to Replace a NextSeq 500/550 Air Filter](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000005604.md)
- [How to create Local Run Manager credentials to perform a NextSeq 500/550 system check in NCS 4.0.X](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000002088.md)
- [How to denature and dilute libraries on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000002064.md)
- [How to enable/disable Illumina Proactive on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000002550.md)
- [How to perform washes on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000003076.md)
- [How to select custom recipes on the NextSeq 500/550 in NextSeq Control Software (NCS) 4.0.X](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000002138.md)
- [How to set up a PhiX validation run on the NextSeq 500/550 in manual mode](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000001532.md)
- [How to set up a Wash on the NextSeq 500/550 Video](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000005864.md)
- [How to set up a run on the NextSeq 500/550 Video](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000005862.md)
- [How to skip the pre run check confirmation on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000003187.md)
- [How to turn on thumbnails for troubleshooting on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000002085.md)
- [How to use a 10X Multiome ATAC Library custom recipe for NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000007480.md)
- [Indexing considerations for NextSeq 500/550 sample sheets](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000002129.md)
- [Instructions for thawing and storing NextSeq 500/550 reagents](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000001974.md)
- [Layout for NextSeq 500/550 flow cells](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000001977.md)
- [Maximum read lengths and number of cycles for different NextSeq 500/550 kits](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000007448.md)
- [NextSeq 500/550 Run Quality and Best Practices Support Webinar Video](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000008045.md)
- [Optical System on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000002076.md)
- [Post Run Automatic Purge for NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000001971.md)
- [Reagent cartridge and buffer layout for NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000002209.md)
- [Recommendations for using custom primers on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000002063.md)
- [Recommended PhiX Loading for a NextSeq 550 Validation Run](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000002068.md)
- [Run folder sizes and disk space on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000007440.md)
- [Software information regarding the NCS 4.0.X upgrade for NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000002375.md)
- [Specifications for the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000001942.md)
- [Temperature control on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000002077.md)
- [Thumbnail image information on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000003084.md)
- [Troubleshooting "Would you like to include the first time setup results…?" on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000002377.md)
- [Upgrading to NextSeq Control Software v4](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000002755.md)
- [Troubleshooting](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list.md)
- [Best practices for NextSeq 500/550 pre run check failures requiring a power cycle](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000005855.md)
- [How to change a NextSeq 500/550 nickname](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000005911.md)
- [How to fix inaccurate or inverted touch screen on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000007990.md)
- [How to perform a NextSeq 500/550 system check](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001883.md)
- [NextSeq 500/550 Control Software v4.0.1 "Invalid Role" error with Local Run Manager v2.4.0](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002190.md)
- [Optimal cluster density guidelines for the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002067.md)
- [Troubleshooting "Local Run Manager Error Row was updated or deleted" error on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000007036.md)
- [Troubleshooting 'Lane Pump not initialized' fluidics related error on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002103.md)
- [Troubleshooting 'Not Found' error message on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001834.md)
- [Troubleshooting Access to the path 'C:\NextSeqCalibration.cfg' is denied error on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000003209.md)
- [Troubleshooting Cannot Update Metrics errors on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002137.md)
- [Troubleshooting Custom Sequencing Primers selected by mistake in manual mode on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002843.md)
- [Troubleshooting Cycle 1 Error, Camera Disabled, Failed to Detect Clusters on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001681.md)
- [Troubleshooting Local Run Manager originally caused by an internal server error on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000007806.md)
- [Troubleshooting NVRAM or Socket Errors on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001391.md)
- [Troubleshooting NextSeq 500/550 (NCS 2.2 or earlier) not showing Planned Run from BaseSpace PrepTab](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000007437.md)
- [Troubleshooting NextSeq 500/550 run stopping with "Local Run Manager Error: Bad Request" error](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002771.md)
- [Troubleshooting Object Reference error on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000007435.md)
- [Troubleshooting Possible Stale Image Data Detected on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001388.md)
- [Troubleshooting RFID errors on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001914.md)
- [Troubleshooting RheodyneValve error on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001389.md)
- [Troubleshooting ThermoCouple\_Side TemperatureProbe not operating as expected on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000007810.md)
- [Troubleshooting Unable to Unload Reagents on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002726.md)
- [Troubleshooting Universal Copy Server error on the NextSeq 500/550 (NCS 4.0+)](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002770.md)
- [Troubleshooting Wash State Unknown in NextSeq 500/550 Control Software (NCS) v4.0.0](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002114.md)
- [Troubleshooting a CavroSyringePump Error on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001380.md)
- [Troubleshooting a Failed Camera Board on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001683.md)
- [Troubleshooting a Failed to Get Temperature error on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001382.md)
- [Troubleshooting a Flow Cell Door error on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000003265.md)
- [Troubleshooting a Flow Cell Registration Failed (Skew) warning on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002053.md)
- [Troubleshooting a NextSeq 500/550 An existing connection was forcibly closed by a remote host error](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002361.md)
- [Troubleshooting a NextSeq 500/550 Cavro Syringe Pump error](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000007432.md)
- [Troubleshooting a RAID error on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001688.md)
- [Troubleshooting a Remote Server Returned An Error on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001812.md)
- [Troubleshooting a Software Shutdown Issue Warning on the NextSeq 500/550 with NCS 4.0.X](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002449.md)
- [Troubleshooting a Specific Camera Disabled Failed to Detect Clusters error on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001379.md)
- [Troubleshooting a buffer cartridge/tray stuck in the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001668.md)
- [Troubleshooting a flow cell registration stall during pre check on first run on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002112.md)
- [Troubleshooting an Async Firmware Operation Error on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001680.md)
- [Troubleshooting an Internal Temperature was Out of Range error on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000007529.md)
- [Troubleshooting an empty output folder on the NextSeq 500/550 NCS 4.0](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000005028.md)
- [Troubleshooting an error related to InstrumentMaintenanceState.xml on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000003516.md)
- [Troubleshooting an imaging system failed pre run check error on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001385.md)
- [Troubleshooting an initialization failure due to Proxy warning on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001684.md)
- [Troubleshooting blank BaseSpace login screen during run setup for Windows 10 instruments (NextSeq 500/550, MiSeq, MiniSeq and Novaseq 6000)](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000003666.md)
- [Troubleshooting flow cell registration failed to find fiducial on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001383.md)
- [Troubleshooting fluidics automation error on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001384.md)
- [Troubleshooting issues unloading reagents on a NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000007434.md)
- [Troubleshooting mid run Camera Disabled error on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001378.md)
- [Troubleshooting no output folder created when set in the Local drive on NextSeq 500/550 (NCS 4.0)](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001685.md)
- [Troubleshooting pre run checks with Temperature sensor check failed: Board 0/1 on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000006373.md)
- [Troubleshooting presence of snr.csv Files in Temp folder on NextSeq 500/550 (NCS 4.0.2)](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000003878.md)
- [Troubleshooting spent reagent cartridge is full on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001392.md)
- [Troubleshooting spent reagent cartridge not detected on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001689.md)


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