Troubleshooting an Async Firmware Operation Error on the NextSeq 500/550

This error is due to a transient communication issue that does not affect run progression or performance.

Possible error messages: "AsyncFirmwareOperationCommand timed out waiting for started after XX:XX:XX." "While waiting on an asynchronous firmware operation: camera bitmask 111111 Originally caused by: Failed XY Stage."

Troubleshooting steps:

1. If the run is continuing, allow the run to complete and monitor.

2. After the run completes, review run performance. See Specifications for the NextSeq 500/550 System.

3. Power cycle NextSeq 500/550.\*

4. If the system initializes, proceed with the next sequencing run.

If the same notification is displayed over 2-3 consecutive runs, contact Illumina Technical Support, for further assistance.

\*For further information, search for Knowledge Base Article How to Perform a NextSeq 550 Power Cycle.

For any feedback or questions regarding this article (Illumina Knowledge Article #1680), contact Illumina Technical Support [e-mail].