# Viral Surveillance Panel (VSP) sequencing and analysis frequently asked questions (FAQs)

\*\*What is the recommended read length for sequencing?\*\*2x150 bp is recommended for both Viral Surveillance Panel (VSP) v1 and v2.

**How many reads per sample are recommended?**

(Note: M = million; PE = Paired End)

VSP v1 and v2 = Minimum 2 M PE reads (1 M clusters) for low complexity samples, or 8 M PE reads (4 M clusters) for high complexity samples (eg, wastewater). Labs may need to optimize reads per sample based on project and data needs.

\*\*How to analyze the data?\*\*VSP v1: [DRAGEN Microbial Enrichment](https://basespace.illumina.com/apps/14527515/DRAGEN-Microbial-Enrichment?preferredversion), [DRAGEN Microbial Enrichment Plus](https://basespace.illumina.com/apps/16156140/DRAGEN-Microbial-Enrichment-Plus?preferredversion), or [DRAGEN Targeted Microbial](https://basespace.illumina.com/apps/16717701/DRAGEN-Targeted-Microbial?preferredversion)\
VSP v2: [DRAGEN Microbial Enrichment Plus](https://basespace.illumina.com/apps/16156140/DRAGEN-Microbial-Enrichment-Plus?preferredversion)

\
\
\ <br>

|                                                                                                                                                                                                                                                                                                                                                                              |
| :--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------: |
| *For any feedback or questions regarding this article (Illumina Knowledge Article #9081), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000009081%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |