# Capture and wash best practices for Illumina RNA Prep with Enrichment (L) Tagmentation

**Capture and wash best practices**

* Verify use of correct bead type: **SMB**
* Use bead handling best practices
* Preheat microheating system and EEW reagent
* **Thermal cycler must run continuously** throughout capture and washes
* Prepare fresh Elution Master Mix (with HP3)
* Be sure to use **heated washes** to remove non-specific binding from the bead

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #3311), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000003311%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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