Capture and wash best practices for Illumina RNA Prep with Enrichment (L) Tagmentation

Capture and wash best practices

• Verify use of correct bead type: SMB

• Use bead handling best practices

• Preheat microheating system and EEW reagent

• Thermal cycler must run continuously throughout capture and washes

• Prepare fresh Elution Master Mix (with HP3)

• Be sure to use heated washes to remove non-specific binding from the beads

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