# Can RSB be used as a substitute for RSB + Tween 20 when sequencing on the NextSeq 1000/2000? Resuspension Buffer (RSB) cannot be used as a substitute for RSB + Tween 20 when sequencing with NextSeq 1000/2000 flow cells. If RSB + Tween 20 is not available, substitute 10 mM Tris-HCl, pH 8.5 with 0.1% Tween 20. \ \ \
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