Illumina DNA Prep with Enrichment third party probe panel requirements
Last updated
Was this helpful?
Last updated
Was this helpful?
Illumina DNA Prep with Enrichment (formerly Nextera Flex for Enrichment) is a targeted enrichment workflow that is compatible with both Illumina and third-party enrichment panels.
Third-Party DNA Probe Panel Requirements
If using third-party biotinylated DNA probe panels with the Illumina DNA Prep with Enrichment workflow, the enrichment panels need to meet the following criteria:
80 bp or 120 bp probe length
Between 500 and 675,000 probes
Single-stranded or double-stranded DNA probes
Total probe input of ≥ 3 pmole for enrichment at plexities from 1-plex to 12-plex
Follow the when using DNA probes.
Third-Party RNA Probe Panel Requirements
If using third-party biotinylated RNA probe panels with the Illumina DNA Prep with Enrichment workflow, the enrichment panels need to meet the following criteria:
120 nt probe length
Between 300,000 and 675,000 probes
Single-stranded
Total probe input of ≥ 3 pmoles for enrichment at plexities from 1-plex to 12-plex
Follow the when using RNA probes.
The use of third-party probe panels with Illumina DNA Prep with Enrichment may require additional optimization and validation, because third-party probe panels have not been validated by Illumina.
For any feedback or questions regarding this article (Illumina Knowledge Article #7483), contact Illumina Technical Support .