# Illumina DNA Prep with Enrichment third party probe panel requirements

Illumina DNA Prep with Enrichment (formerly Nextera Flex for Enrichment) is a targeted enrichment workflow that is compatible with both Illumina and third-party enrichment panels.

**Third-Party DNA Probe Panel Requirements**

If using third-party biotinylated DNA probe panels with the Illumina DNA Prep with Enrichment workflow, the enrichment panels need to meet the following criteria:

* 80 bp or 120 bp probe length
* Between 500 and 675,000 probes
* Single-stranded or double-stranded DNA probes
* Total probe input of ≥ 3 pmole for enrichment at plexities from 1-plex to 12-plex

Follow the [Illumina DNA Prep with Enrichment Reference Guide](https://support.illumina.com/sequencing/sequencing_kits/illumina-dna-prep-with-enrichment/documentation.html) when using DNA probes.

The use of third-party probe panels with Illumina DNA Prep with Enrichment may require additional optimization and validation, because third-party probe panels have not been validated by Illumina.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #7483), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000007483%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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