# Illumina DNA Prep with Enrichment third party probe panel requirements Illumina DNA Prep with Enrichment (formerly Nextera Flex for Enrichment) is a targeted enrichment workflow that is compatible with both Illumina and third-party enrichment panels. **Third-Party DNA Probe Panel Requirements** If using third-party biotinylated DNA probe panels with the Illumina DNA Prep with Enrichment workflow, the enrichment panels need to meet the following criteria: * 80 bp or 120 bp probe length * Between 500 and 675,000 probes * Single-stranded or double-stranded DNA probes * Total probe input of ≥ 3 pmole for enrichment at plexities from 1-plex to 12-plex Follow the [Illumina DNA Prep with Enrichment Reference Guide](https://support.illumina.com/sequencing/sequencing_kits/illumina-dna-prep-with-enrichment/documentation.html) when using DNA probes. The use of third-party probe panels with Illumina DNA Prep with Enrichment may require additional optimization and validation, because third-party probe panels have not been validated by Illumina. \ \ \
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