Illumina DNA Prep with Enrichment third party probe panel requirements
Illumina DNA Prep with Enrichment (formerly Nextera Flex for Enrichment) is a targeted enrichment workflow that is compatible with both Illumina and third-party enrichment panels.
Third-Party DNA Probe Panel Requirements
If using third-party biotinylated DNA probe panels with the Illumina DNA Prep with Enrichment workflow, the enrichment panels need to meet the following criteria:
80 bp or 120 bp probe length
Between 500 and 675,000 probes
Single-stranded or double-stranded DNA probes
Total probe input of ≥ 3 pmole for enrichment at plexities from 1-plex to 12-plex
Follow the Illumina DNA Prep with Enrichment Reference Guide when using DNA probes.
Third-Party RNA Probe Panel Requirements
If using third-party biotinylated RNA probe panels with the Illumina DNA Prep with Enrichment workflow, the enrichment panels need to meet the following criteria:
120 nt probe length
Between 300,000 and 675,000 probes
Single-stranded
Total probe input of ≥ 3 pmoles for enrichment at plexities from 1-plex to 12-plex
Follow the Nextera Flex for Enrichment for RNA Probes Demonstrated Protocol when using RNA probes.
The use of third-party probe panels with Illumina DNA Prep with Enrichment may require additional optimization and validation, because third-party probe panels have not been validated by Illumina.
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