# Reagent stability and thawing for the iSeq 100

## **Thawing**

After receiving an iSeq 100 reagent shipment, store the reagent cartridge(s) in the freezer at -20°C to -25°C with the **arrow pointing up for at least 24 hours before thawing for use**. This is **required**.

There are multiple ways to thaw the reagent cartridge.

* If thawed in a room temperature water bath (20°C to 25°C) for a minimum of 6 hours, the cartridge is stable for up to 18 hours in the water bath.\*
* If thawed in a refrigerator (2°C to 8°C) for a minimum of 36 hours, the cartridge is stable for up to 1 week in the refrigerator.
* If thawed in room temperature air (20°C to 25°C) for a minimum of 9 hours, the cartridge is stable up to 18 hours in room temperature air.
* **Do not thaw iSeq 100 consumables above room temperature.**

\*If planning on using the water bath thaw method, store the reagent cartridge at -20°C for at least one day before use. If the reagent cartridge is moved directly from dry ice used during shipping to a water bath thaw, this can heat shock the fluidics systems and cause total run failure.

Improper thawing can cause quality issues or complete cluster failures.

![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-ca330564880204707a235b8a9eae5689653a1fac%2Fimage1.png?alt=media\&token=9421d1cf-5e83-40d7-b310-203b66c2e61f)

**Flow cell Preparation**

Remove the flow cell from the fridge, set aside the unopened package and allow it to come to room temperature for 10-15 minutes.

## **Reagent Stability**

Reagents must be used immediately after thawing (within the recommended time ranges described above), and re-freezing reagents is **not** recommended or supported. The longest thaw time is 1 week at 4°C.

## **Flow cell Stability**

Once the flow cell is opened and removed from the package, it must be used within 24 hours.

See the [iSeq 100 Sequencing System Guide](https://support.illumina.com/downloads/iseq-100-system-guide.html) for additional information.

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