Can a NovaSeq 6000 4 lane Xp kit be used for 2 lane NovaSeq 6000 flow cells?

Yes. Although the NovaSeq 6000 Xp 4 lane kits include a manifold for a 4 lane flow cell, because lane inlets and outlets are positioned consistently, the manifold can also be used for a 2 lane flow cell. The reagents are also compatible, although the full reagent volumes in the 4 lane Xp kit will not be used for a 2 lane cell flow cell, as lower volumes are used for 2 lane flow cells.

For any feedback or questions regarding this article (Illumina Knowledge Article #7916), contact Illumina Technical Support techsupport@illumina.com.

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