Final library QC and run setup with the Respiratory Virus Oligos Panel v2 (RVOP v2)

What is the recommended read length? A 2x75 bp read length is recommended when sequencing Respiratory Virus Oligos Panel v2 (RVOP v2) libraries. This shorter read length takes into account the potentially shorter average size of libraries created with samples of variable quality.

What is the recommended read depth? 1 million reads per sample are recommended as a starting point.

Are there public datasets available? Yes, the following public datasets are available in BaseSpace Sequence Hub. (Note: BaseSpace login is required to access the following links.)

iSeq 100: Illumina RNA Prep with Enrichment and RVOPv2 (SARS-CoV-2 Synthetic RNA Control) Run Link Project Link

MiniSeq: Illumina RNA Prep with Enrichment and RVOPv2 (SARS-CoV-2 Synthetic RNA Control) Run Link Project Link

MiSeq: Illumina RNA Prep with Enrichment and RVOPv2 (SARS-CoV-2 Synthetic RNA Control) Run Link Project Link

NextSeq 550: Illumina RNA Prep with Enrichment and RVOPv2 (SARS-CoV-2 Synthetic RNA Control). Run Link Project Link

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