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Troubleshooting MiSeq washes taking extended time to complete
If a MiSeq wash is taking too long to complete, it is possible the MiSeq has had a communication error and the wash has stalled.
- 1.Confirm which wash was intended and how long ago it was started. Verify time in the wash is longer than what is expected for the type of wash.
- Post-run wash: 20 minutes or 30 minutes, if using the template line wash option.
- Maintenance wash: 90 minutes (3 steps - 30 minutes each step).
- Standby wash: 120 minutes (2 steps - 60 minutes each step).
- 2.Power cycle* the MiSeq.
- If the control software is responsive, exit the wash and shut down the MiSeq.
- If the control software is not responsive, open the task manager to end the task for MiSeq control software via the following pathway: Task Manager > Processes > MiSeqSoftware.exe > End Task.
- Open the task manager by right-clicking in the taskbar and select task manager or by pressing CTRL +ALT+DEL and select task manager.
- Shut down MiSeq through the Windows start menu, and toggle the power switch at the back of the instrument to the OFF position. Leave powered down for a full minute.
- 3.After turning the instrument back on, if the instrument initializes without errors, repeat the wash that was in progress and monitor the time to complete.
*For further information, search for Knowledge Base articles Wash best practices for MiSeq, How to Shut down or power cycle the MiSeq, and How To Power Cycle the MiSeq Video.