TruSeq Small RNA alternative T4 RNA Ligase 2, Deletion Mutant supplier

The TruSeq Small RNA reference guide lists T4 RNA Ligase 2, Deletion Mutant #LR2D1132K and LR2D11310K as user-supplied consumables. However, Lucigen (formerly, Epicentre) has discontinued this product.

Illumina has identified New England Biolab T4 RNA Ligase 2, truncated (Catalog #M0242) as a suitable alternative to the Lucigen T4 RNA Ligase, Deletion Mutant enzyme. NEB M0242 is now recommended for use in the TruSeq Small RNA assay. This enzyme should be used as a 1:1 substitution without any protocol changes.

For any feedback or questions regarding this article (Illumina Knowledge Article #8542), contact Illumina Technical Support [e-mail].