How to Perform a NextSeq 500/550 Power Cycle

NextSeq 500/550 Power Cycle Instructions

  • From the instrument home screen, select Manage Instrument.

  • Select System Shut down Options.

  • Select Shut Down (this will shut down software and turn off instrument power).

  • Wait two minutes before turning on the instrument from the button in front.

  • Wait for software initialization back to the Home Screen.

For NextSeq Control Software (NCS) 4.0/Windows 10 there other options available if the Control Software is not accessible:

  1. Within Windows, use the Start > Illumina > NextSeq Shut down option. This will mimic shutting down through the Control Software and is the best alternate recommendation.

  2. Open the Windows command prompt and use the shutdown /s command.

    1. If this option is used, toggle the switch on the back of the instrument and wait 2 minutes before powering back up.

Note: If the NextSeq Control Software or the Windows OS is frozen/nonresponsive and there are no available options to shut down, hold the front power button down for 10-20 seconds to turn off the instrument. Toggle the power switch on the back of the instrument and wait 2 minutes before powering the instrument back on.

For any feedback or questions regarding this article (Illumina Knowledge Article #1972), contact Illumina Technical Support techsupport@illumina.com.

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