# How to set the output location in the NovaSeq 6000 Control Software

The NovaSeq 6000 platform requires the designation of an output folder path to a network location prior to the start of a sequencing run in Manual mode. For more information on how to configure the output folder path in File-Based mode, refer to the [NovaSeq 6000 Sequencing System Guide](https://support.illumina.com/sequencing/sequencing_instruments/novaseq-6000/documentation.html).

**Important Notes:**

* If the run uses BaseSpace Sequence Hub for both analysis and storage, setting a network output folder location is optional. In this scenario a warning will be presented "The output folder must exist and cannot be on C:\ D:\ or Z:\ drives". After importing a valid Sample Sheet, the "Review" option will be available to move forward with setting up the run.
* A mapped network drive should be configured by your network administrator.
* The UNC path for the network drive is required for setting the output location. Using a mapped letter, such as Y:, results in an Invalid Drive error message.
* Example of a UNC path: \servername\share-directory

To specify a default output location in the NovaSeq Control Software:

1. From the **Main Menu**, select **Settings**.
2. Select **Manual**.
3. Select **Browse** (next to the Output Folder field).
4. Type or copy and paste the UNC path into the address bar.
5. Select **Enter** on keyboard, and input network credentials if prompted.
6. Designate the desired folder and click **Select Folder**.
7. Select **Save**.

Once the default output location has been designated, the output folder field will automatically populate on the Run Setup screen for future runs. To change the output location for a single run, follow steps 3-7 on the Run Setup screen.

For additional information on networking requirements or run setup procedures, refer to the following resources:

[NovaSeq 6000 Sequencing System Guide](https://support.illumina.com/sequencing/sequencing_instruments/novaseq-6000/documentation.html)\
[NovaSeq 6000 Sequencing System Site Prep Guide](https://support.illumina.com/downloads/novaseq-site-prep-guide-1000000019360.html)

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #1530), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000001530%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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