How to set the output location in the NovaSeq 6000 Control Software

The NovaSeq 6000 platform requires the designation of an output folder path to a network location prior to the start of a sequencing run in Manual mode. For more information on how to configure the output folder path in File-Based mode, refer to the NovaSeq 6000 Sequencing System Guide.

Important Notes:

  • If the run uses BaseSpace Sequencing Hub for both analysis and storage, setting a network output folder location is optional.

  • A mapped network drive should be configured by your network administrator.

  • The UNC path for the network drive is required for setting the output location. Using a mapped letter, such as Y:, results in an Invalid Drive error message.

  • Example of a UNC path: \servername\share-directory

To specify a default output location in the NovaSeq Control Software:

  1. From the Main Menu, select Settings.

  2. Select Manual.

  3. Select Browse (next to the Output Folder field).

  4. Type or copy and paste the UNC path into the address bar.

  5. Select Enter on keyboard, and input network credentials if prompted.

  6. Designate the desired folder and click Select Folder.

  7. Select Save.

Once the default output location has been designated, the output folder field will automatically populate on the Run Setup screen for future runs. To change the output location for a single run, follow steps 3-7 on the Run Setup screen.

For additional information on networking requirements or run setup procedures, refer to the following resources:

NovaSeq 6000 Sequencing System Guide NovaSeq 6000 Sequencing System Site Prep Guide

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