# How to dilute custom primers for use on the iSeq 100?

Dilute custom primers with HT1\* buffer (catalog number #20015892). Insert read primers (Read 1 and Read 2) must be diluted to 0.3 µM in 140 µl. Index read primers must be diluted to 0.6 µM in 140 µl.

For more information, see the Custom Primers section of the [iSeq 100 Sequencing System Guide](https://support.illumina.com/downloads/iseq-100-system-guide.html).

\*For more information on HT1, search for Knowledge Base article **Hybridization Buffer (HT1) and Resuspension Buffer (RSB) alternatives and purchasing availability**.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #6503), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000006503%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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