# TruSeq Stranded RNA kit storage and freeze/thaw recommendations

**How should the Resuspension Buffer (RSB) be stored?**\
The Resuspension Buffer should be stored at -15 to -25°C when first received. After the initial thaw the reagent can be stored at 2 to 8°C for use throughout the protocol.

**Can the adapter plate undergo multiple freeze/thaws? How many times?**\
Yes, the adapter plate can undergo freeze/thawing up to 4 times. More freeze thaws of the plates may impair assay performance.

**When generating sample numbers below than the full amount that the kit supports, how should the core reagents be aliquoted or freeze/thawed?** **How many samples per aliquot?**\
The reagents can undergo up to four freeze/thaw cycles. If additional freeze/thaw cycles are required, aliquot the reagents after the initial thaw and refreeze the remaining aliquots to avoid excessive freeze/thaw cycles. When reagents are aliquoted, there may not be enough reagents for the full number of reactions over multiple uses.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #1152), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000001152%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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