TruSeq Stranded RNA Kit FAQs: storage and freeze/thaw recommendations

How should the Resuspension Buffer (RSB) be stored? The Resuspension Buffer should be stored at -15 to -25°C when first received. After the initial thaw the reagent can be stored at 2 to 8°C for use throughout the protocol.

Can I freeze/thaw the adapter plate? How many times? Yes, the adapter plate can undergo freeze/thawing up to 4 times. More freeze thaws of the plates may impair assay performance.

When generating samples less than the full amount of the kit how should the core reagents be aliquoted or freeze/thawed? How many samples per aliquot? If the entire reagent in a tube will not be consumed in one use, we recommend dispensing the reagent into aliquots (a minimum of 24 sample preps/aliquot) after the initial thaw and refreeze the remaining aliquots to avoid excessive freeze/thaw cycles. However, if aliquoted, there may not be enough reagents for the full number of reactions over multiple uses.

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