Optimal cluster density guidelines for the NextSeq 500/550

The recommended raw cluster density for NextSeq 500/550 High-Output and Mid-Output runs is 170-220k/mm2 for well-balanced, base diverse libraries (eg, whole genome libraries, RNA-seq libraries, etc.).

For further information, search for Knowledge Base articles:

• Cluster density guidelines for Illumina sequencing platforms.

• How to achieve more consistent cluster density on Illumina Sequencing Platforms.

For any feedback or questions regarding this article (Illumina Knowledge Article #2067), contact Illumina Technical Support [e-mail].