Technical resources for the iSeq 100

A comprehensive list of resources needed to set up, operate, maintain, and troubleshoot the iSeq 100 are on the iSeq 100 Support page. Specific documentation links can be found below.* System Setup Poster Contains information for first-time instrument setup. It is included with the shipment of the instrument.

• Illumina CMOS Chip and One-Channel SBS Chemistry tech note Provides an explanation of single-channel chemistry and how it compares to two- and four-channel chemistries.

• QC and rebalancing of single-cell gene expression libraries using the iSeq 100 System Assess key metrics of multiplexed single-cell libraries to evaluate quality and enable accurate rebalancing before high-depth runs on the NextSeq 550 or NovaSeq 6000 Systems.

• Sequencing Library QC with the iSeq 100 System This application note details how to use the iSeq 100 System to sequence libraries as a quality control (QC) step before high throughput sequencing.

• iSeq 100 System Specification Sheet Contains basic information including output and time specifications for the iSeq100.

• iSeq 100 System Specification Page Provides the data quality and yield specifications for the iSeq 100.

• iSeq 100 Sequencing System Guide Contains dilution and loading instructions, a workflow overview, maintenance procedures, troubleshooting resources, software restriction policy information, and FAQs.

• Online Training Courses Includes video courses on unboxing and installing your instrument, how to start a run, and setting up / viewing analysis results on Local Run Manager. To access additional online training courses, go to Illumina.com -> Support -> Webinars and Trainings -> Online Courses -> Sequencing.

• Safety Data Sheets Safety Data Sheets contain information to help with appropriate reagent disposal practices. To obtain this information, enter the kit catalog number, language, and region/country, then select Search.

Additional technical resources are available 24/7 at the iSeq 100 Sequencing System support website.

For any feedback or questions regarding this article (Illumina Knowledge Article #2345), contact Illumina Technical Support [e-mail].