# Technical resources for the iSeq 100

A comprehensive list of resources needed to set up, operate, maintain, and troubleshoot the iSeq 100 are on the iSeq 100 Support page. Specific documentation links can be found below.\* [System Setup Poster](http://emea.support.illumina.com/downloads/iseq-100-setup-poster-1000000035963.html?langsel=/de/)\
Contains information for first-time instrument setup. It is included with the shipment of the instrument.

* [Illumina CMOS Chip and One-Channel SBS Chemistry tech note](https://support.illumina.com/content/dam/illumina-marketing/documents/products/techspotlights/cmos-tech-note-770-2013-054.pdf)\
  Provides an explanation of single-channel chemistry and how it compares to two- and four-channel chemistries.
* [QC and rebalancing of single-cell gene expression libraries using the iSeq 100 System](https://www.illumina.com/content/dam/illumina-marketing/documents/systems/iseq/single-cell-library-qc-app-note-770-2019-029.PDF)\
  Assess key metrics of multiplexed single-cell libraries to evaluate quality and enable accurate rebalancing before high-depth runs on the NextSeq 550 or NovaSeq 6000 Systems.
* [Sequencing Library QC with the iSeq 100 System](https://www.illumina.com/content/dam/illumina-marketing/documents/products/appnotes/novaseq-qc-iseq-app-note-770-2018-019.pdf)\
  This application note details how to use the iSeq 100 System to sequence libraries as a quality control (QC) step before high throughput sequencing.
* [iSeq 100 System Specification Sheet](https://support.illumina.com/content/dam/illumina-marketing/documents/products/datasheets/iseq100-sequencing-system-spec-sheet-770-2017-020.pdf)\
  Contains basic information including output and time specifications for the iSeq100.
* [iSeq 100 System Specification Page](https://www.illumina.com/systems/sequencing-platforms/iseq/specifications.html)\
  Provides the data quality and yield specifications for the iSeq 100.
* [iSeq 100 Sequencing System Guide](https://emea.support.illumina.com/downloads/iseq-100-system-guide.html)\
  Contains dilution and loading instructions, a workflow overview, maintenance procedures, troubleshooting resources, software restriction policy information, and FAQs.
* [Online Training Courses](http://emea.support.illumina.com/sequencing/sequencing_instruments/iseq-100/training.html)\
  Includes video courses on unboxing and installing your instrument, how to start a run, and setting up / viewing analysis results on Local Run Manager. To access additional online training courses, go to Illumina.com -> Support -> Webinars and Trainings -> Online Courses -> Sequencing.
* [Safety Data Sheets](https://support.illumina.com/sds.html)\
  Safety Data Sheets contain information to help with appropriate reagent disposal practices. To obtain this information, enter the kit catalog number, language, and region/country, then select **Search**.

Additional technical resources are available 24/7 at the [iSeq 100 Sequencing System support website](https://support.illumina.com/sequencing/sequencing_instruments/iseq-100.html).

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #2345), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000002345%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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