How to use the Resources page to add custom reference files for Run Planning for NovaSeq X/X Plus runs

While other instrument types and BaseSpace apps allow reference files to be selected directly from Projects, the NovaSeq X/X Plus requires that files be uploaded to the Resources page on BaseSpace.

1. To access the Resources page, select the profile icon at the top right of BaseSpace and select Resources.

2. Custom library and index kits must be uploaded to the Library Prep Kits and Index Adapter Kits sections respectively:

https://basespace.illumina.com/resources/library-prep-kits

https://basespace.illumina.com/resources/index-adapter-kits

3. Any auxiliary reference files for downstream analysis, such as a target BED file or Panel of Normals CNV, must be uploaded to the Reference Files page:

https://basespace.illumina.com/resources/reference-files

The File Type must match the exact field that the reference file is being used for. For example, a file uploaded as a BedFile cannot be used in the QC Coverage BED filed of DRAGEN Germline. It must be uploaded as a QcCoverageBedFile file type:

4. Custom genomes built with DRAGEN Reference Builder on BaseSpace must be linked to the Genomes page from the BaseSpace Project in order to be usable in NovaSeq X/X Plus Run Planning:

https://basespace.illumina.com/resources/genomes

For any feedback or questions regarding this article (Illumina Knowledge Article #8500), contact Illumina Technical Support [e-mail].