> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/library-preparation/dna-library-prep.md).

# DNA Library Prep

- [FAQ](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list.md)
- [Analysis FAQ for Illumina DNA PCR-Free libraries](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000003440.md)
- [Bead size selection FAQ for Illumina DNA PCR-Free](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000003466.md)
- [Can FFPE input be used with the Illumina DNA PCR-Free library preparation kit?](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000003437.md)
- [Consumables used in Illumina DNA PCR-Free workflow](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000003448.md)
- [DNA Input Requirements for Illumina DNA PCR-Free Library Prep](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000003432.md)
- [Denature and dilution FAQ for Illumina DNA PCR-Free](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000003444.md)
- [Differences between the Illumina DNA Prep (M) Tagmentation and Nextera XT kits](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000001256.md)
- [Does the Flex Lysis Kit require the purchase of additional Illumina reagents?](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000007307.md)
- [How does the Illumina/Nextera tagmentation enzyme make sure that both p5 and p7 adapters are added?](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000005831.md)
- [How to quantify and what is the expected yield for Illumina DNA PCR-Free libraries?](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000003477.md)
- [Illumina DNA PCR-Free custom sequencing primer FAQ](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000003445.md)
- [Index selection and FAQ for Illumina DNA PCR-Free](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000003471.md)
- [Input DNA requirements for TruSeq DNA PCR-Free library preparation](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000001182.md)
- [Input FAQ for Illumina DNA PCR-Free library preparation](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000003438.md)
- [Instrument, flow cell, and reagent requirement FAQ for Illumina DNA PCR-Free libraries](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000003443.md)
- [Library QC for the Illumina DNA Prep (M) Tagmentation Kit](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000001258.md)
- [Library storage FAQ for Illumina DNA PCR-Free libraries](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000003468.md)
- [Product page and support page for Illumina DNA Prep, (M) Tagmentation](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000005311.md)
- [Product page and support page links for Illumina DNA Prep with Exome 2.5 Enrichment](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000007302.md)
- [Qualification/QC FAQ for Illumina DNA PCR-Free libraries](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000003478.md)
- [Saliva collection FAQ for Illumina DNA PCR-Free library preparation](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000003434.md)
- [Saliva processing FAQ for Illumina DNA PCR-Free library preparation](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000003435.md)
- [Sample Input for Illumina DNA Prep, (M) Tagmentation Library Preparation Kit](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000001262.md)
- [Sample batching with Illumina DNA PCR-Free Prep](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000003464.md)
- [Samples per sequencing run and coverage FAQ for Illumina DNA PCR-Free](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000003442.md)
- [Size selection and clean-up considerations for TruSeq DNA PCR-Free library preparation](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000002777.md)
- [Standard vs low input workflow selection FAQ for Illumina DNA PCR-Free prep](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000003460.md)
- [Using dried blood spots as input into the Illumina DNA PCR-Free library prep kit questions](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000003436.md)
- [What is the Illumina Lysis Kit, and when should it be used?](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000005931.md)
- [When and how to quantify Illumina DNA PCR-Free libraries](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000003476.md)
- [Which Nextera index kits come in plate or tube format?](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000002331.md)
- [Whole blood input requirements for Illumina DNA PCR-Free](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000003433.md)
- [Reference Material](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-reference_material-list.md)
- [Considerations before starting the Illumina DNA PCR-Free library preparation](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-reference_material-list/000003430.md)
- [Considerations for choosing an Illumina DNA PCR-Free library preparation workflow](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-reference_material-list/000005264.md)
- [Illumina DNA PCR-Free Tagmentation Introduction Support Webinar Video](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-reference_material-list/000008037.md)
- [Illumina DNA Prep Tagmentation Introduction Support Webinar Video](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-reference_material-list/000008038.md)
- [Library quantification and manual normalization of Nextera XT libraries](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-reference_material-list/000001145.md)
- [Nextera XT Library Preparation tips and troubleshooting technical support note](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-reference_material-list/000006093.md)
- [Nextera XT Sample Preparation and Usage Support Webinar Video](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-reference_material-list/000008054.md)
- [Nextera XT input considerations](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-reference_material-list/000001023.md)
- [Optimizing coverage for targeted resequencing](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-reference_material-list/000001409.md)
- [Plate layout for Nextera DNA Combinatorial Dual (CD) indexes](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-reference_material-list/000002339.md)
- [Product page and support page for TruSeq DNA Nano](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-reference_material-list/000005222.md)
- [Product page and support page for TruSeq DNA PCR-Free](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-reference_material-list/000005223.md)
- [Product page and support page links for Nextera XT](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-reference_material-list/000005164.md)
- [Sequencing information for Illumina DNA Prep (M) Tagmentation libraries](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-reference_material-list/000001263.md)
- [What reagents are available for ATAC-Seq library preparation?](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-reference_material-list/000001817.md)
- [双链DNA文库浓度的计算：从质量浓度(ng/µl)转化为摩尔浓度(nM)的换算公式](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-reference_material-list/000007413.md)
- [Troubleshooting](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-troubleshooting-list.md)
- [Overtagmentation of Nextera XT libraries](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-troubleshooting-list/000001054.md)
- [Undertagmentation of Nextera XT libraries](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-troubleshooting-list/000001148.md)


---

# Agent Instructions
This documentation is published with GitBook. GitBook is the documentation platform designed so that both humans and AI agents can read, navigate, and reason over technical content effectively. Learn more at gitbook.com.

## Querying This Documentation
If you need additional information that is not directly available in this page, you can query the documentation by asking a question.

Perform an HTTP GET request on the following URL with the `ask` and `goal` query parameters:

```
GET https://knowledge.illumina.com/library-preparation/dna-library-prep.md?ask=<question>&goal=<user_goal>
```

`ask` is the immediate question: it should be specific, self-contained, and written in natural language.
`goal` is what the user is ultimately trying to achieve, the reason they need the answer. Sharing it helps GitBook give you a better, more relevant answer. A goal is most helpful when it describes the outcome the user wants rather than restating the question. For example, with `ask=how do I create an API token`, a goal like `automate deployments from our CI pipeline` lets GitBook tailor the answer to that use case.

The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.

Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.