What is the correct format for DRAGEN Array IDAT CSV Sample Sheet?

When using DRAGEN Array (Cloud or Local) analysis, providing a sample sheet is optional. A sample sheet enables users to analyze samples located in different directories and select specific samples for analysis rather than processing every sample on a BeadChip.

The DRAGEN Array IDAT CSV Sample Sheet is a CSV-formatted input file that utilizes a couple required fields for sample lookup. These fields associate a sample identifier with the corresponding Sentrix Barcode and position on the BeadChip.

For DRAGEN Array Local workflows, the required lookup fields are:

SentrixBarcode_A**:** BeadChip barcode

SentrixPosition_A**:** BeadChip position

Figure 1. Example of a DRAGEN Array Local IDAT CSV Sample Sheet.

For DRAGEN Array Cloud workflows, the required lookup fields are:

beadChipName**:** BeadChip barcode

sampleSectionName

Figure 2. Example of a DRAGEN Array Cloud IDAT CSV Sample Sheet.

The DRAGEN Array CSV Sample Sheet format differs from the GenomeStudio CSV Sample Sheet format. It uses a simplified structure and includes only the information required for DRAGEN Array analysis workflows. In contrast, the GenomeStudio sample sheet may contain additional columns and data fields that are intended for use with GenomeStudio software.

Although the two sample sheet formats differ, the recommendations for formatting 12-digit BeadChip barcodes apply to both DRAGEN Array and GenomeStudio sample sheets.

Note: Beginning with DRAGEN Array v1.3, backward compatibility with the GenomeStudio CSV Sample Sheet format was introduced for DRAGEN Array Local CLI Analysis workflows. This compatibility does not apply to all DRAGEN Array workflows.

References:

• Is a sample sheet required to run DRAGEN Array?

• How to avoid sample sheet formatting errors when creating a GenomeStudio project

• Troubleshooting DRAGEN Array Cloud error Sample sheet missing required columns)

• DRAGEN Array Sample Sheet Templates

• DRAGEN Array User Guide under section Input Files

• How to format file path for DRAGEN Array IDAT sample sheet and GTC sample sheet for Windows OS

For any feedback or questions regarding this article (Illumina Knowledge Article #6290), contact Illumina Technical Support [e-mail].