How to Perform a System Check on the MiniSeq

The MiniSeq System Check process requires the following.

• One wash flow cell (a previously used flow cell; this wash flow cell can be either a mid output or high output used flow cell. ).

• One wash cartridge filled with 40 ml lab-grade water (loaded in well 40 as indicated below).

  

To perform the system check on the MiniSeq:

1. Navigate to the Home screen in the MiniSeq Control Software (MnCS).

2. Select Manage Instrument→System Check. a. MnCS automatically closes to allow the MiniSeq Service Software (MnSS) to launch.

3. Perform the following steps (the instrument does not prompt this).

a. Load a wash flow cell (used flow cell).

b. Empty the spent reagent bottle and return it to the instrument.

c. Load a wash tray with 40 ml lab-grade water in the wash reservoir, position #40. (Note: The MnSS may present the prompt "Fill in BB3 with DI water prior the test. Re-insert wash tray, then press OK". The BB3 position is the same as well position #40).

1. The following tests are pre-selected (the unchecked tests are those performed by Field Service Engineers only).

Motion: FCLM & FAM, Stage Test Optics: Flow cell Registration Fluidics: Valve response, Pressure decay, Flow rate Thermal: Fans, Thermal probes

1. Select Next to begin the system checks.

The system checks require manual input about 5-10 minutes after the check start during the pressure decay test.

The checks take approximately 20 minutes to complete.

Once the test completes, a read-out of each test showing Pass or Fail result appears on the screen. If any of the tests fail, contact Illumina Technical Support at [e-mail] and provide the System Check results from D:\Illumina\SystemCheckResults\SystemCheckResultsDetails.csv. After reviewing the results, select Exit to close the MiniSeq Service Software and return to MiniSeq Control Software.

For any feedback or questions regarding this article (Illumina Knowledge Article #2040), contact Illumina Technical Support [e-mail].