How to Perform a System Check on the MiniSeq
The MiniSeq System Check process requires the following.
One wash flow cell (a previously used flow cell; this wash flow cell can be either a mid output or high output used flow cell. ).
One wash cartridge filled with 40 ml lab-grade water or 0.05% Tween in lab-grade water (loaded in well 40 as indicated below).
To perform the system check on the MiniSeq:
Navigate to the Home screen in the MiniSeq Control Software (MnCS).
Select Manage Instrument→System Check. a. MnCS will close automatically to allow the MiniSeq Service Software (MNSS) to launch.
Perform the following steps (the instrument will not prompt this).
a. Load a wash flow cell (used flow cell).
b. Empty the spent reagent bottle and return it to the instrument.
c. Load a wash tray with 40 ml lab-grade water to the wash reservoir (position #40).
The following tests are pre-selected (the unchecked tests are those performed by Illumina Engineers only).
Motion: FCLM & FAM, Stage Test Optics: Flow cell Registration Fluidics: Valve response, Pressure decay, Flow rate Thermal: Fans, Thermal probes
Select Next to begin the system checks.
The system checks will require manual input about 5-10 minutes into the check during the pressure decay test.
The checks take approximately 20 minutes to run.
Once the test is complete, a read-out of each test showing pass or fail status appears on the screen.
If all tests pass, the instrument is functioning properly, and any issue is likely consumable- or library-related.
If any of the tests fail, this can indicate a hardware issue. Contact Illumina Technical Support ([email protected]) and provide the System Check results from D:\Illumina\SystemCheckResults\SystemCheckResultsDetails.csv.
After reviewing the results, select Exit to close the MiniSeq Service Software and return to MiniSeq Control Software.
For any feedback or questions regarding this article (Illumina Knowledge Article #2040), contact Illumina Technical Support [email protected].
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