How to Perform a System Check on the MiniSeq

The MiniSeq System Check process requires the following.

• One wash flow cell (a previously used flow cell; this wash flow cell can be either a mid output or high output used flow cell. ).

• One wash cartridge filled with 40 ml lab-grade water or 0.05% Tween in lab-grade water (loaded in well 40 as indicated below).

  

To perform the system check on the MiniSeq:

1. Navigate to the Home screen in the MiniSeq Control Software (MnCS).

2. Select Manage Instrument→System Check. a. MnCS will close automatically to allow the MiniSeq Service Software (MNSS) to launch.

3. Perform the following steps (the instrument will not prompt this).

a. Load a wash flow cell (used flow cell).

b. Empty the spent reagent bottle and return it to the instrument.

c. Load a wash tray with 40 ml lab-grade water to the wash reservoir (position #40).

1. The following tests are pre-selected (the unchecked tests are those performed by Illumina Engineers only).

Motion: FCLM & FAM, Stage Test Optics: Flow cell Registration Fluidics: Valve response, Pressure decay, Flow rate Thermal: Fans, Thermal probes

1. Select Next to begin the system checks.

The system checks will require manual input about 5-10 minutes into the check during the pressure decay test.

The checks take approximately 20 minutes to run.

Once the test is complete, a read-out of each test showing pass or fail status appears on the screen.

1. If all tests pass, the instrument is functioning properly, and any issue is likely consumable- or library-related.

2. If any of the tests fail, this can indicate a hardware issue. Contact Illumina Technical Support ([e-mail]) and provide the System Check results from D:\Illumina\SystemCheckResults\SystemCheckResultsDetails.csv.

After reviewing the results, select Exit to close the MiniSeq Service Software and return to MiniSeq Control Software.

For any feedback or questions regarding this article (Illumina Knowledge Article #2040), contact Illumina Technical Support [e-mail].