How to configure External Storage locations on the NovaSeq X/X Plus

The NovaSeq X/X Plus requires a network storage location to serve as the output folder for sequencing data. The instrument stores data in a temporary folder on the instrument while Universal Copy Service (UCS) transfers the data to the intended output folder. If there are disruptions to the data transfer, UCS will resume the transfer once the network is back online.

Prior to run setup, the network storage must be configured through the External Storage options within the Control Software. See the following instructions for setting this up.

Before starting this process, collect the following information from the local IT team.

• Network protocol type, either Server Message Block (SMB) or Network File System (NFS).

• Fully qualified Universal Naming Convention (UNC) path to the share folder.

• Network username and credentials (usually a Service Account).

• The network domain if the server is joined to a domain.

Add a Network Drive to External Storage

1. Select the instrument icon to open the global navigation menu.

2. Select Settings, and then select External Storage.

3. Select Add network storage.

4. Select the network drive type, either SMB or NFS.

5. Enter the following information:

   1. Server location (as the UNC Path).

   2. [Optional] Domain.

   3. Network User name and password.

6. If using SMB protocol for network storage, select a file encryption option. Using encryption is recommended.

7. Select Test configuration to test the network storage connection.

8. If the test completes successfully, select Save.

If there are any issues with completing this process, contact Illumina Technical Support.

Specify External Storage as the Output Folder

After adding a network drive to External Storage, use the following instructions to configure the Output Folder on the network drive.

1. Select the instrument icon to open the global navigation menu.

2. Select Settings, and then select External Storage.

3. Select a server location from the list of network locations added to External Storage.

4. If an output folder has been added, select Edit folders, and then Add folder.

5. If an output folder has not been added, select Add folder.

6. Select a server location from the drop-down list, and then select one of the available folders.

7. Enter a folder nickname.

8. Enter a name for the output folder.

9. Select Save.

10. Optional: Remove output folders by selecting Remove on the Edit folders screen.

For any feedback or questions regarding this article (Illumina Knowledge Article #7775), contact Illumina Technical Support [e-mail].