# TruSeq Stranded RNA library preparation input requirements

**Can the TruSeq Stranded RNA kits generate libraries from FFPE samples or from only high quality total RNA?**\
The TruSeq Stranded mRNA kit supports high quality RNA (minimum RIN of 8) as input for sample prep as the protocol relies on a polyA tail purification step. The TruSeq Stranded Total Stranded RNA kit with both Ribo-Zero kit configurations (Human/Mouse/Rat and Gold) is compatible with FFPE samples.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #1154), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000001154%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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