IDT for Illumina TruSeq Unique Dual (UD) Index kit FAQs for kit configurations and usage

The full support page for the IDT for Illumina TruSeq Unique Dual (UD) Index kits is available here.

How many index combinations are available? The IDT for Illumina TruSeq UD Indexes come in two versions: 24 unique dual indexes (repeated 4 times in a 96-well plate) and 96 unique dual indexes. Index plate layouts are on the page here.

What are the catalog numbers for the IDT for Illumina TruSeq UD Indexes, and which library preparation kits are compatible?

• IDT for Illumina - TruSeq DNA UD Indexes are compatible with TruSeq DNA PCR Free, TruSeq DNA Nano, TruSeq DNA Exome library prep kit.

  • IDT for Illumina - TruSeq DNA UD Indexes v2 (96 Indexes, 96 Samples) 20040870

• IDT for Illumina - TruSeq RNA UD Indexes are compatible with TruSeq Stranded mRNA, and TruSeq Stranded Total RNA library prep kits.

  • IDT for Illumina - TruSeq RNA UD Indexes v2 (96 Indexes, 96 Samples) 20040871

Which Illumina sequencing platforms are compatible with IDT for Illumina TruSeq UD indexes? IDT for Illumina TruSeq UD indexes are compatible with all Illumina sequencing platforms.

Do any of index sets collide or match any of other Illumina index kits? The IDT for Illumina TruSeq UD indexes are not designed to be used with any other Illumina index sets.

Are there considerations for low-plexity pooling? Yes, there are recommended index combinations for 2-plex though 96-plex within the plate. For more information, see the Index Adapter Pooling Guide.

What are the storage and shipping conditions of these index kits? The reagents are shipped on dry ice and must be stored at -25°C to -15°C upon arrival.

What is the fill volume (μl) in each well of an IDT for Illumina TruSeq UD Index plates? There are 10 μl of dual index in each well with some overage to account for storage and pipetting.

Are the IDT for Illumina TruSeq UD Indexes compatible with all products from Illumina? No; consult the library preparation kit product page for index kit compatibility.

Do the indexes need to be diluted prior to use? No, do not dilute the indexes before use; the indexes are supplied at the working concentration.

Does the index volume used per sample need to be adjusted when using the IDT for Illumina TruSeq UD Indexes? No, there is no change to the index volume required for compatible library preparation workflows (2.5 µl used per sample).

Will using the IDT for Illumina TruSeq UD indexes change the final library QC traces? Libraries prepared with the IDT for Illumina TruSeq UD Indexes are expected to migrate similarly to the libraries prepared with Combinatorial Dual (CD) or single index adapters.

For any feedback or questions regarding this article (Illumina Knowledge Article #1878), contact Illumina Technical Support [e-mail].