Troubleshooting no output folder created when set in the Local drive on NextSeq 500/550 (NCS 4.0)

Users setting up output folders in a disk drive of the NextSeq 500/550 (Local D:\ drive or an external hard drive such as USB) may have the sequencing runs completed with all the data present in the Temp folder, but no run folder created in the output location. This can be seen in runs performed in either Manual or Local Run Manager modes.

Troubleshooting steps:

• Manually transfer run data files from original Temp folder (D:\Illumina\NextSeq Control Software Temp[Run ID]) to the output directory.

• Change Output location for subsequent run to a two folder levels deep in the desired directory (eg, D:\Illumina\NextSeq Output).

• If the issue persists, contact Illumina Technical Support for further assistance.

For any feedback or questions regarding this article (Illumina Knowledge Article #1685), contact Illumina Technical Support [e-mail].