# Number of libraries per AmpliSeq for Illumina Library PLUS kit for custom and community panels

Each AmpliSeq custom and community panel contains at least one pool of primer pairs, though each panel may have up to four primer pools to create overlapping amplicons for complete coverage of a target. Panel configurations with three or four primer pools require more DNA and reagents than panels with one or two primer pools.

Refer to the following table for the total number of libraries supported by an AmpliSeq Library PLUS kit based on the number of primer pools.

| AmpliSeq Library PLUS Configuration | Total Number of Libraries Supported (DNA and RNA) |         |     |
| ----------------------------------- | ------------------------------------------------- | ------- | --- |
| 1 or 2 Pools                        | 3 Pools                                           | 4 Pools |     |
| 24 reactions                        | 24                                                | 16      | 12  |
| 96 reactions                        | 96                                                | 64      | 48  |
| 384 reactions                       | 384                                               | 256     | 192 |

\
\
\ <br>

|                                                                                                                                                                                                                                                                                                                                                                              |
| :--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------: |
| *For any feedback or questions regarding this article (Illumina Knowledge Article #2098), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000002098%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


---

# Agent Instructions: Querying This Documentation

If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.

Perform an HTTP GET request on the current page URL with the `ask` query parameter:

```
GET https://knowledge.illumina.com/library-preparation/ampliseq/library-preparation-ampliseq-reference_material-list/000002098.md?ask=<question>
```

The question should be specific, self-contained, and written in natural language.
The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.

Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.