Reference Material

Library Preparation > Illumina Single Cell RNA Prep > Reference Material

• 0.8X bead cleanup to remove adapter dimers from Illumina Single Cell 3’ RNA Prep libraries

• Assessing cDNA QC in Illumina Single Cell 3’ RNA Prep

• Assessing library QC in Illumina Single Cell 3’ RNA Prep

• Best Practices for Nuclei Isolation with alternative nuclei isolation protocols for Illumina Single Cell

• Best practices for cell preparation for Illumina Single Cell prep

• Best practices for using Illumina Single Cell Nuclei Isolation Kits for Illumina Single Cell kits

• Cell input and volume loading recommendations for Illumina Single Cell 3’ RNA Prep

• Differences between V4.0PLUS PIPseq and Illumina Single Cell (formerly PIPseq V)

• Extending cDNA storage time in Illumina Single Cell 3' RNA Prep

• Illumina Single Cell 3' RNA Preparation library sequencing recommendations and guidelines

• Illumina Single Cell 3’ RNA Prep cDNA QC best practices

• Illumina Single Cell 3’ RNA Prep library QC best practices

• Representative cDNA QC traces for Illumina Single Cell 3’ RNA Prep

• Representative library QC traces for Illumina Single Cell 3’ RNA Prep

• Sample Enrichment Best Practices for Illumina Single Cell 3' RNA Prep

• Selecting cycle numbers for High and Low RNA sample types with Illumina Single Cell RNA prep kits

• Shelf life of Illumina Single Cell 3' RNA Prep kits

• Supplemental Enrichment and Amplification (SEA) primer design for Illumina Single Cell RNA Kits

• The importance of pilot studies for Illumina Single Cell RNA Preparation prior to workflow changes