FAQ

Library Preparation > RNA Library Prep > FAQ

• Are technical replicates needed for TruSeq Stranded RNA Seq experiments?

• Are the IDT for Illumina RNA Index Anchors the same in every well of the plate?

• COVIDSeq timing and logistics

• Can vortexing damage cells/nuclei when generating emulsions in the Illumina Single Cell workflow?

• Differences with TruSeq Stranded RNA LS and HS protocols and single and dual index adapters

• Directional sequencing and strandedness for TruSeq Stranded Total RNA and mRNA libraries

• Frequently asked questions regarding the Ribo Zero Plant and Globin kits

• General information on Ribo Zero Plus Microbiome

• Guidance for RNA isolated from PAXgene Blood RNA kit

• Illumina RNA Prep Tagmentation (L) with Enrichment General FAQs

• Illumina Stranded Total RNA Prep Ligation with Ribo Zero Plus FAQs

• Illumina Stranded mRNA Prep Ligation General FAQs

• Illumina Stranded mRNA Prep Ligation Input FAQs

• Illumina Stranded mRNA Prep Ligation Sequencing FAQs

• Input FAQs for the Illumina TruSight RNA Pan Cancer library preparation kit

• Input recommendations and considerations for COVIDSeq (RUO) library preparation

• Overview of the IDT for Illumina RNA UD Index Sets, Ligation

• Product page and support page links for Illumina Stranded Total RNA with Ribo Zero Plus

• Product page and support page links for TruSeq Stranded Total RNA

• Protocol FAQs for Illumina Stranded Total RNA Prep Ligation with Ribo Zero Plus

• Ribo Zero Plus rRNA Depletion Kit FAQs

• Sequencing FAQs for Illumina Stranded Total RNA Prep Ligation with Ribo Zero Plus

• Sequencing TruSeq Small RNA libraries

• Sequencing depth and read length considerations for TruSeq Stranded RNA libraries

• Shelf life for the COVIDSeq library preparation kit

• TruSeq RNA v2 product and support page links

• TruSeq Small RNA Product and Support Pages

• TruSeq Small RNA alternative T4 RNA Ligase 2, Deletion Mutant supplier

• TruSeq Small RNA input best practices

• TruSeq Stranded RNA Kit FAQs: AMPure clean up and library size

• TruSeq Stranded RNA kit storage and freeze/thaw recommendations

• TruSeq Stranded RNA library preparation input requirements

• TruSeq Stranded mRNA Product and Support Pages

• What are the main differences between TruSeq Stranded and Illumina Stranded kits?

• What volume of TruSeq RNA UD indexes should be used during TruSeq Stranded RNA library preparation?

• mRNA purification and rRNA removal information for TruSeq Stranded RNA workflows