Troubleshooting an initialization failure due to Proxy warning on the NextSeq 500/550

The NextSeq Control Software (any version) may fail to initialize due to a Proxy System blocking access to IP addresses required for hardware communications. Internal Network Interface Card (NIC) and the system's Main Printed Circuit Assembly board must be given proxy "exceptions" to operate normally.

The error messages may appear as follows:

• "Failed to communicate with firmware via FTP at 'XXX.XX.XXX.X'. This may be caused by incorrect Proxy Settings. If the instrument is using Proxy Servers for LAN, ensure the IP Address..."

• "The remote server returned an error: (500) Internal Server Error."

  

Troubleshooting steps:

1. Exit the NextSeq Control Software.

2. Navigate to the Control Panel > Internet Options > Connections Tab > LAN settings.

3. If "Use a proxy server..." is checked, select Advanced.
4. Enter the appropriate IP address in the Exceptions field. Separate each IP with semicolons.

• 192.168.113.2

• Depending on which instrument/control software, add the following IP address as well:

  • Instrument	IP Address
    Windows 7 (NCS v2.2 or below)	192.168.113.5
    Windows 10 (NCS 4.0+ without Airflow upgrade)	192.168.113.5
    Windows 10 (NCS 4.0+ with Airflow upgrade)	192.168.113.3

  • If in doubt, contact Illumina Technical Support for additional assistance.

  

5. Select OK and close all control panel boxes.

6. Restart the NextSeq Control Software.

For any feedback or questions regarding this article (Illumina Knowledge Article #1684), contact Illumina Technical Support [e-mail].